Vliianie temperatury i stepeni gidratatsii na podvizhnost' spinovykh metok v poverkhnostnykh sloiakh belkov.

Translated title of the contribution: Effect of temperature and degree of hydration on the mobility of spin labels in surface layers of proteins

O. V. Belonogova, E. N. Frolov, N. V. Illiustrov, G. I. Likhtenshteǐn

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The mobility of separate sites of the water-protein matrix depending on temperature and degree of hydration has been investigated by means of spin labels covalently attached to surface layers of proteins (alpha-chymotrypsin and human serum albumin) and also by a spin probe in a hydrophobic "pocket" of human serum albumin. The results obtained are compared with the data on the mobility of gamma-resonance labels (57Fe) firmly bound with the protein matrix in the same samples. At certain temperature and degree of hydration both spin and gamma-resonance label show an increase in mobility. With the degree of hydration increasing one may observe a simultaneous increase in energy and in entropy of activation: rotatory diffusion of spin labels, i. e., a compensation effect takes place which confirms the concept expressed earlier that cooperation of water-protein interactions is the main reason of CEF. It should be noted that at P/PS greater than 0.8 the values of delta E =7 divided by 10 kcal/mole, and delta S not equal to = 9 divided by 11 e. e. are specific to glycerol-like systems, i. e., under these conditions (P/Ps greater than 0.8) the water-protein layer has glycerol-like properties.

Translated title of the contributionEffect of temperature and degree of hydration on the mobility of spin labels in surface layers of proteins
Original languageRussian
Pages (from-to)567-576
Number of pages10
JournalMolekulyarnaya Biologiya
Volume13
Issue number3
StatePublished - 1 May 1979

ASJC Scopus subject areas

  • General Medicine

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