Why does Coomassie Brilliant Blue R interact differently with different proteins? A partial answer

M. Tal, A. Silberstein, E. Nusser

Research output: Contribution to journalArticlepeer-review

229 Scopus citations

Abstract

Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their capacity for interaction with Coomassie Brilliant Blue G-250 (Coomassie G) in solution. Scatchard analysis showed that the number of Coomassie R ligands bound to each protein molecule is approximately proportional to the number of positive charges on the protein, about 1.5-3 dye molecules/charge.

Original languageEnglish
Pages (from-to)9976-9980
Number of pages5
JournalJournal of Biological Chemistry
Volume260
Issue number18
StatePublished - 1 Dec 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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