Abstract
Agrobacterium is the only known bacterium capable of natural DNA transfer into a eukaryotic host. The genes transferred to host plants are contained on a T-DNA (transferred DNA) molecule, the transfer of which begins with its translocation, along with several effector proteins, from the bacterial cell to the host-cell cytoplasm. In the host cytoplasm, the T-complex is formed from a single-stranded copy of the T-DNA (T-strand) associated with several bacterial and host proteins and it is imported into the host nucleus via interactions with the host nuclear import machinery. Once inside the nucleus, the T-complex is most likely directed to the host genome by associating with histones. Finally, the chromatin-associated T-complex is uncoated from its escorting proteins prior to the conversion of the T-strand to a double-stranded form and its integration into the host genome.
Original language | English |
---|---|
Pages (from-to) | 333-345 |
Number of pages | 13 |
Journal | Canadian Journal of Physiology and Pharmacology |
Volume | 84 |
Issue number | 3-4 |
DOIs | |
State | Published - 1 Mar 2006 |
Externally published | Yes |
Keywords
- Agrobacterium
- Integration
- Nuclear import
- T-DNA
ASJC Scopus subject areas
- Physiology
- Pharmacology
- Physiology (medical)