Will you let me use your nucleus? How Agrobacterium gets its T-DNA expressed in the host plant cell

Benoît Lacroix, Jianxiong Li, Tzvi Tzfira, Vitaly Citovsky

Research output: Contribution to journalReview articlepeer-review

31 Scopus citations

Abstract

Agrobacterium is the only known bacterium capable of natural DNA transfer into a eukaryotic host. The genes transferred to host plants are contained on a T-DNA (transferred DNA) molecule, the transfer of which begins with its translocation, along with several effector proteins, from the bacterial cell to the host-cell cytoplasm. In the host cytoplasm, the T-complex is formed from a single-stranded copy of the T-DNA (T-strand) associated with several bacterial and host proteins and it is imported into the host nucleus via interactions with the host nuclear import machinery. Once inside the nucleus, the T-complex is most likely directed to the host genome by associating with histones. Finally, the chromatin-associated T-complex is uncoated from its escorting proteins prior to the conversion of the T-strand to a double-stranded form and its integration into the host genome.

Original languageEnglish
Pages (from-to)333-345
Number of pages13
JournalCanadian Journal of Physiology and Pharmacology
Volume84
Issue number3-4
DOIs
StatePublished - 1 Mar 2006
Externally publishedYes

Keywords

  • Agrobacterium
  • Integration
  • Nuclear import
  • T-DNA

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