TY - JOUR
T1 - Yak rumen fluid inoculum increases biogas production from sheep manure substrate
AU - Sohail, Muhammad
AU - Khan, Alam
AU - Badshah, Malik
AU - Degen, Allan
AU - Yang, Guo
AU - Liu, Hu
AU - Zhou, Jianwei
AU - Long, Ruijun
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (U21A20250), and the Key Research and Development Program for International Cooperation of Gansu Province, China (21YF5WA117). Additionally, we appreciate Dr. Malik Badshah for providing the research facilities at the Sustainable Bioenergy and Biorefinery Laboratory, Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Funding Information:
This work was supported by the National Natural Science Foundation of China ( U21A20250 ), and the Key Research and Development Program for International Cooperation of Gansu Province, China (21YF5WA117). Additionally, we appreciate Dr. Malik Badshah for providing the research facilities at the Sustainable Bioenergy and Biorefinery Laboratory, Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/10/1
Y1 - 2022/10/1
N2 - Hydrolytic bacteria are essential for the degradation of lignocellulose to produce biogas and organic fertilizers. In this study, sheep manure was used as substrate, and sheep manure slurry, yak rumen fluid and slurry from a biogas reactor (SBR) were used as inocula in single-stage anaerobic digestion. The SBR and rumen fluid inocula increased biogas production by 23% and 43%, respectively, when compared to solely sheep manure in the single-stage anaerobic digestion. The two-stage anaerobic digestion, with yak rumen fluid as inoculum in the hydrolytic reactor, increased the biogas production by 59, 86, and 58% compared with the control. Microbial analysis of the effluent revealed that yak rumen fluid contained hydrolytic bacteria such as Proteiniphilum, Jeotgalibaca, Fermentimonas, and Atopostipes to enhance the degradation of sheep manure and increase biogas production. It was concluded that yak rumen fluid, rich in hydrolytic bacteria, increases the degradability of sheep manure and improves production of volatile fatt acids and biogas.
AB - Hydrolytic bacteria are essential for the degradation of lignocellulose to produce biogas and organic fertilizers. In this study, sheep manure was used as substrate, and sheep manure slurry, yak rumen fluid and slurry from a biogas reactor (SBR) were used as inocula in single-stage anaerobic digestion. The SBR and rumen fluid inocula increased biogas production by 23% and 43%, respectively, when compared to solely sheep manure in the single-stage anaerobic digestion. The two-stage anaerobic digestion, with yak rumen fluid as inoculum in the hydrolytic reactor, increased the biogas production by 59, 86, and 58% compared with the control. Microbial analysis of the effluent revealed that yak rumen fluid contained hydrolytic bacteria such as Proteiniphilum, Jeotgalibaca, Fermentimonas, and Atopostipes to enhance the degradation of sheep manure and increase biogas production. It was concluded that yak rumen fluid, rich in hydrolytic bacteria, increases the degradability of sheep manure and improves production of volatile fatt acids and biogas.
KW - Hydrolytic bacteria
KW - Rumen fluid
KW - Sheep manure
KW - Single-stage anaerobic digestion
KW - Two-stage anaerobic digestion
UR - http://www.scopus.com/inward/record.url?scp=85136476969&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2022.127801
DO - 10.1016/j.biortech.2022.127801
M3 - Article
C2 - 35995345
AN - SCOPUS:85136476969
SN - 0960-8524
VL - 362
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 127801
ER -
