Yak rumen fluid inoculum increases biogas production from sheep manure substrate

Muhammad Sohail, Alam Khan, Malik Badshah, Allan Degen, Guo Yang, Hu Liu, Jianwei Zhou, Ruijun Long

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Hydrolytic bacteria are essential for the degradation of lignocellulose to produce biogas and organic fertilizers. In this study, sheep manure was used as substrate, and sheep manure slurry, yak rumen fluid and slurry from a biogas reactor (SBR) were used as inocula in single-stage anaerobic digestion. The SBR and rumen fluid inocula increased biogas production by 23% and 43%, respectively, when compared to solely sheep manure in the single-stage anaerobic digestion. The two-stage anaerobic digestion, with yak rumen fluid as inoculum in the hydrolytic reactor, increased the biogas production by 59, 86, and 58% compared with the control. Microbial analysis of the effluent revealed that yak rumen fluid contained hydrolytic bacteria such as Proteiniphilum, Jeotgalibaca, Fermentimonas, and Atopostipes to enhance the degradation of sheep manure and increase biogas production. It was concluded that yak rumen fluid, rich in hydrolytic bacteria, increases the degradability of sheep manure and improves production of volatile fatt acids and biogas.

Original languageEnglish
Article number127801
JournalBioresource Technology
StatePublished - 1 Oct 2022


  • Hydrolytic bacteria
  • Rumen fluid
  • Sheep manure
  • Single-stage anaerobic digestion
  • Two-stage anaerobic digestion

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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