TY - JOUR
T1 - Yeast surface display of dehydrogenases in microbial fuel-cells
AU - Gal, Idan
AU - Schlesinger, Orr
AU - Amir, Liron
AU - Alfonta, Lital
N1 - Funding Information:
Research was supported by an Israel Science Foundation (ISF) Program ( 232/13 ), L.A. The helpful assistance of Mr. Alon Szczupak, Mr. Yehonatan Ravenna and Mr. Ron Feiner is gratefully acknowledged.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Two dehydrogenases, cellobiose dehydrogenase from Corynascus thermophilus and pyranose dehydrogenase from Agaricus meleagris, were displayed for the first time on the surface of Saccharomyces cerevisiae using the yeast surface display system. Surface displayed dehydrogenases were used in a microbial fuel cell and generated high power outputs. Surface displayed cellobiose dehydrogenase has demonstrated a midpoint potential of − 28 mV (vs. Ag/AgCl) at pH = 6.5 and was used in a mediator-less anode compartment of a microbial fuel cell producing a power output of 3.3 μW cm− 2 using lactose as fuel. Surface-displayed pyranose dehydrogenase was used in a microbial fuel cell and generated high power outputs using different substrates, the highest power output that was achieved was 3.9 μW cm− 2 using D-xylose. These results demonstrate that surface displayed cellobiose dehydrogenase and pyranose dehydrogenase may successfully be used in microbial bioelectrochemical systems.
AB - Two dehydrogenases, cellobiose dehydrogenase from Corynascus thermophilus and pyranose dehydrogenase from Agaricus meleagris, were displayed for the first time on the surface of Saccharomyces cerevisiae using the yeast surface display system. Surface displayed dehydrogenases were used in a microbial fuel cell and generated high power outputs. Surface displayed cellobiose dehydrogenase has demonstrated a midpoint potential of − 28 mV (vs. Ag/AgCl) at pH = 6.5 and was used in a mediator-less anode compartment of a microbial fuel cell producing a power output of 3.3 μW cm− 2 using lactose as fuel. Surface-displayed pyranose dehydrogenase was used in a microbial fuel cell and generated high power outputs using different substrates, the highest power output that was achieved was 3.9 μW cm− 2 using D-xylose. These results demonstrate that surface displayed cellobiose dehydrogenase and pyranose dehydrogenase may successfully be used in microbial bioelectrochemical systems.
KW - Cellobiose dehydrogenase
KW - Enzymatic fuel cells
KW - Microbial fuel cells
KW - Pyranose dehydrogenase
KW - Yeast surface display
UR - http://www.scopus.com/inward/record.url?scp=84989945925&partnerID=8YFLogxK
U2 - 10.1016/j.bioelechem.2016.07.006
DO - 10.1016/j.bioelechem.2016.07.006
M3 - Article
AN - SCOPUS:84989945925
SN - 1567-5394
VL - 112
SP - 53
EP - 60
JO - Bioelectrochemistry
JF - Bioelectrochemistry
ER -