Yeast surface display of dehydrogenases in microbial fuel-cells

Idan Gal, Orr Schlesinger, Liron Amir, Lital Alfonta

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Two dehydrogenases, cellobiose dehydrogenase from Corynascus thermophilus and pyranose dehydrogenase from Agaricus meleagris, were displayed for the first time on the surface of Saccharomyces cerevisiae using the yeast surface display system. Surface displayed dehydrogenases were used in a microbial fuel cell and generated high power outputs. Surface displayed cellobiose dehydrogenase has demonstrated a midpoint potential of − 28 mV (vs. Ag/AgCl) at pH = 6.5 and was used in a mediator-less anode compartment of a microbial fuel cell producing a power output of 3.3 μW cm− 2 using lactose as fuel. Surface-displayed pyranose dehydrogenase was used in a microbial fuel cell and generated high power outputs using different substrates, the highest power output that was achieved was 3.9 μW cm− 2 using D-xylose. These results demonstrate that surface displayed cellobiose dehydrogenase and pyranose dehydrogenase may successfully be used in microbial bioelectrochemical systems.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalBioelectrochemistry
Volume112
DOIs
StatePublished - 1 Dec 2016

Keywords

  • Cellobiose dehydrogenase
  • Enzymatic fuel cells
  • Microbial fuel cells
  • Pyranose dehydrogenase
  • Yeast surface display

Fingerprint

Dive into the research topics of 'Yeast surface display of dehydrogenases in microbial fuel-cells'. Together they form a unique fingerprint.

Cite this